Mushroom Science Experiments: Tissue Cultures

After you've finished harvesting your mushrooms, you might be wondering what to do now. 

Oyster Mushrooms lend themselves to tissue cultures, a fun way to expand your oyster mushroom mycellium onto new substrate.

A tissue culture is where you take a small piece of mushroom tissue and use it to grow an entire new organism. 

Oyster Mushrooms grow on cellulose. You can find numerous sources of cellulose around your house including: corrugated cardboard, straw, toilet paper, and coffee grounds.



 What You Need: 

  • Small Piece of Oyster Mushroom
  • Cellulose - we recommend starting with square of corrugated cardboard cut into 4" squares.



1. Bring a pot of water to a boil.

2. Place cellulose into the boiling water for 3 - 5 minutes.

3. Remove cellulose to a pan or plate to cool.

4. Peel open cellulose and place a small piece of Oyster Mushroom on the cardboard.

5. Place the cellulose with the mushroom piece into a plastic bag and zip closed.

6. Place in a warm spot for 7 - 10 days. Check for mycellial growth.

7. Open daily for 3 weeks. Small mushrooms may begin to grow. 


Photos of the Process: