Mushroom Science Experiments - Tissue Cultures

After you've finished harvesting your mushrooms, you might be wondering what to do now? Oyster mushrooms lend themselves to tissue cultures, a fun way to expand your oyster mushroom mycellium onto new substrate. 

A tissue culture is where you take a small piece of mushroom tissue and use it to grow an entire new organism. 

Oyster mushrooms grow on cellulose and you can find numerous sources of cellulose around your house, including: corrugated cardboard, straw, toilet paper, and coffee grounds.

What you need: 

Small piece of oyster mushroom 

Cellulose - we recommend starting with square of corrugated cardboard cut into 4" squares.

Steps:

1. Boil a pot of water

2. Place cellulose into the boiling water for 3 - 5 minutes

3. Remove cardboard/cellulose to a pan or plate to cool

4. Peel open cardboard and place a small piece of oyster mushroom in the cardboard.

5. Place the cardboard with the mushroom piece into a plastic bag and zip closed

6. Place in a warm spot for 7 - 10 days. Check for mycellial growth.

7. Open daily for 3 weeks. Small mushrooms may begin to grow.